Table of Contents

Introduction.-Helicobacter pylori: An Overview.-Perspectives on Methodology for in vitro Culture of Helicobacter pylori.-Successful Culture Techniques for Helicobacter Species : General Culture Techniques for Helicobacter pylori.-Successful Culture Techniques for Helicobacter Species: Establishing H. pylori Cultures From Infected Rodents.-Successful Culture Techniques for Helicobacter Species: Verification of Helicobacter Identity Using 16S rRNA Gene Sequence Analysis.-The Helicobacter pylori cag Pathogenicity Island.-Genetic Manipulation of a Naturally Competent Bacterium, Helicobacter pylori.-A Method for Short-term Culture of Human Gastric Epithelial Cells to Study the Effects of Helicobacter pylori.-Cell Culture Based Assays to Test for Bacterial Adherence and Internalization.-Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence.-Rodent Models of Helicobacter Infection, Inflammation and Disease.-Bacterial Culture and Inoculation of Mice (Simple Infection).-Adoptive Transfer of Splenocytes to Immunocompromised Mice.-Isolation of Gastric Lamina Propria Leukocytes (LPL).-Delayed-type Hypersensitivity Determination.-Necropsy, Blood, Tissue Collection & mRNA Isolation for Detection of Host Cytokine Gene Expression.-Animal Models of Helicobacter Induced Disease – Methods to Successfully Infect the Mouse.-Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice.-Mouse Models of Helicobacter-induced Gastric Cancer: Use of Cocarcinognes.-Gastric Helicobacter spp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections.-Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models.-Innate Immune Responses to Helicobacter pylori Infection- An Overview.-Methods for In vivo and In vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection.-Techniques for Following Labeled Cells In vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-galactosidase Detection.-In vivo Measurement of Helicobacter pylori Infection.A Method for Short-term Culture of Human Gastric Epithelial Cells to Study the Effects of Helicobacter pylori.-Cell Culture Based Assays to Test for Bacterial Adherence and Internalization.-Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence.-Rodent Models of Helicobacter Infection, Inflammation and Disease.-Bacterial Culture and Inoculation of Mice (Simple Infection).-Adoptive Transfer of Splenocytes to Immunocompromised Mice.-Isolation of Gastric Lamina Propria Leukocytes (LPL).-Delayed-type Hypersensitivity Determination.-Necropsy, Blood, Tissue Collection & mRNA Isolation for Detection of Host Cytokine Gene Expression.-Animal Models of Helicobacter Induced Disease – Methods to Successfully Infect the Mouse.-Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice.-Mouse Models of Helicobacter-induced Gastric Cancer: Use of Cocarcinognes.-Gastric Helicobacter spp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections.-Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models.-Innate Immune Responses to Helicobacter pylori Infection-An Overview.-Methods for In vivo and In vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection.-Techniques for Following Labeled Cells In vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-galactosidase Detection.-In vivo Measurement of Helicobacter pylori Infection.