Table of Contents

Fluorescence Microscopy.- Need for Standardization of Fluorescence Measurements from the Instrument Manufacturer's View.- Characterization and Calibration in Wide Field and Sectioned Fluorescence Microscopy SIPcharts.- Quantitative Fluorescence Microscopy: Considerations and Controls.- Comparability of Fluorescence Microscopy Data and Need for Instrument Characterization of Spectral Scanning Microscopes.- Fluorescence Lifetime Imaging Microscopy: Quality Assessment and Standards.- Single Molecule Spectroscopy.- State of the Art and Novel Trends in Fluorescence Correlation Spectroscopy.- Single Molecule Spectroscopy: Instrumentation and Multiparameter Detection.- Fluorescence-Based Microarray Technology: Applications, Future Trends, and Need for Standardization.- DNA Microarrays: Applications, Future Trends, and the Need for Standardization.- Comparability of Microarray Experiments from the Instrument and the Sample Site and Approaches Towards Standardization.- Microarray Technology: Unresolved Issues and Future Challenges from a Regulatory Perspective.- Protein Arrays and Fluorescence Detection: Applications and Limitations.- Flow Cytometry.- Flow Cytometry: Instrumentation, Applications, Future Trends and Limitations.- Flow Cytometry Quality Assurance.- Approaches to Quantitation in Flow Cytometry.- Fluorescence Immunoassays.- Immunoassays: Basic Concepts, Physical Chemistry and Validation.- Time-Resolved Fluorometric Immunoassays; Instrumentation, Applications, Unresolved Issues and Future Trends.- Particle-Based Assays: Applications and Unresolved Issues.- Advances in Fluorescence Enzyme Detection Methods.- Quantitative PCR.- Quantitative Real-Time PCR: Fluorescent Probe Options and Issues.- Fluorescence In Situ Hybridization and Immunohistochemistry.- CellularBioimaging in Fluorescent Cancer Biomarker Evaluation: Validation, Technologies and Standards Development.- Fluorescence Technologies in Biomedical Diagnostics.- Fluorescence Techniques in Biomedical Diagnostics: Instrumentation, Analysis and Unresolved Issues.- In-vivo Fluorescence Imaging: Applications, Future Trends & Approaches to Standardization.