Acknowledgements; Abbreviations; 1. Introduction; 2. Collection and examination of human semen; 2A. Standard procedures; 2B. Optional tests; 2C. Research tests; 3. Sperm preparation techniques; 4. Quality control in the andrology laboratory; 5. Sperm-cervical mucus interactions; Appendices. IA. Reference values of semen variables; IB. Nomenclature for some semen variables; II. Safety guidelines for the andrology laboratory; III. Methods for detecting leucocytes; IV. Sperm vitality techniques; V. Papanicolau staining procedures modified for spermatozoa; VI. Shorr staining procedure for sperm morphology; VII. Rapid staining procedure for sperm morphology; VIII. Immunobead test; IX. Mixed antiglobulin reaction test (MAR test); X. Measurement of zinc in seminal plasma; XI. Measurement of fructose in seminal plasma; XII. Measurement of neutral a-glucosidase in seminal plasma; XIII. Sample instructions and record form for semen analysis; XIV. Computer-aided sperm analysis (CASA); XV. Protocols for the zona-free hamster test; XVI. Measurement of reactive oxygen species generated by sperm suspensions; XVII. Induced acrosome reaction assay; XVIII. Sperm preparation techniques; XIX. Quality control (QC) procedures; XX. Sample record form for cervical mucus scoring and postcoital test; XXI. Sperm-cervical mucus interaction: the capillary tube test; XXII. Basic requirements for an andrology laboratory; Bibliography; Index.
The definitive and essential source of reference for all laboratories involved in the analysis of human semen.
'This is a handy little techniques book for the
andrology-spermatology laboratory. Methods for sperm evaluation and
mucus systems to enable equation between laboratories/technicians
are included … the WHO have provided an updated methods book
readily sought by those interested in sperm and their morphology
and function.' Journal of Obstetrics and Gynaecology
'This manual is comprehensive and easy to understand. The
methodologies are very detailed, from initial morphology
assessments to sperm counts and smear preparations for further
microscopy. This is a good manual and I would personally recommend
it to any laboratory that performs semen analysis. It would also be
a good reference tool in forensic and artificial insemination
laboratories.' Isaac Aliche, Canadian Journal of Medical Laboratory
Science
'The WHO manual should have a prominent place in the benches of all
laboratories dealing with human semen and on the shelves of
clinicians dealing with subfertile couples … The new fourth
edition, or the 'gold book' as I am sure it will be called, will
then continue to be the benchmark for setting more rigorous
standards in the future.' J. Diane Critchlow, Human Fertility
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