Table of Contents

I. Laying the Foundation 1. An Overview of Biotechnology 2. Biotechnology and Society 3. Genes, Genetics, and Geneticists 4. An Overview of Molecular Biology 5. Applying Molecular Biology: Recombinant Technology II. Classroom Activities A. DNA Structure and Function 6. Constructing a Paper Helix 7. DNA Replication 8. From Genes to Proteins 9. Sizes of the Escherichia coli and Human Genomes 10. Extraction of Bacterial DNA B. Manipulation and Analysis of DNA 11. DNA Scissors: Introduction to Restriction Enzymes 12. DNA Goes to the Races 13. Restriction Analysis of Lambda DNA 14. Recombinant Paper Plasmids 15. Restriction Analysis Challenge Worksheets 16. Detection of Specific DNA Sequences: Hybridization Analysis 17. DNA Sequencing 18. The Polymerase Chain Reaction: Paper PCR 19. Analyzing Genetic Variation: DNA Typing 20. A Mixup at the Hospital 21. A Paternity Case 22. The Case of the Bloody Knife C. Transfer of Genetic Informtion 23. Transformation of Escherichia coli 24. Conjugative Transfer of Antibiotic Resistance in Escherichia coli 25. Transduction of an Antibiotic Resistance Gene 26. Agrobacterium tumefaciens: Nature's Plant Genetic Engineer D. Bioinformatics and Evolutionary Analysis of Proteins 27. Testing for Amylase Activity 28. Electrophoresis of Amylase Samples 29. Constructing an Amylase Evolutionary Tree 30. Bioinformatics E. Molecular Biology and Genetics 31. An Adventure in Dog Hair, Part I 32. An Adventure in Dog Hair, Part II: Yellow Labs 33. Human Molecular Genetics Part III. Societal Issues 34. Science, Technology, and Society 35. Weighing Technology's Risks and Benefits 36. Debating the Risks of Biotechnology 37. A Decision-Making Model for Bioethical Issues 38. Bioethics Case Study: Gene Therapy 39. Bioethics Case Study: Genetic Screening 40. Careers in Biotechnology Part IV Appendixes Glossary