M. Berrios, Introduction.
Cell Fractionation Preparation and Characterization of Probes:
M. Berrios, Isolation and Characterization of Nuclear Pore
Complex-Lamina Fractions from Vertebrates.
P.A. Fisher,Preparation and Characterization of Karyoskeletal
Protein-Enriched Fractions from Drosophila melanogaster.
J.P. Aris, Isolation of Nuclei and Nucleoli from Yeast
Saccharomyces cerevisiae.
Structural Analysis of the Interphase Nucleus:
M.R. Paddy, Qualitative and Quantitative Approaches to the Spatial
Localization of Nuclear Proteins with the Fluorescence Light
Microscope.
U. Kubitscheck and R. Peters, Localization of Single Nuclear Pore
Complexes by Confocal Laser Scanning Microscopy and Analysis of
their Distribution.
A.S. Belmont, Nuclear Ultrastructure Transmission Electron
Microscopy and Image Analysis.
T.D. Allen, S.A. Rutherford, G.R. Bennion, M.W. Goldberg, and E.
Kiseleva, Three-Dimensional Surface Structure Analysis of the
Nucleus.
J. Hainfeld and J. Wall, Scanning Transmission Electron Microscopy
of Nucleic Acid-Protein Complexes.
Chromatin and Sub-Nuclear Structures:
C.L. Woodcock and R.A. Horowitz, Chromatin and Nucleosomal
Assemblies.
A.F. Dernburg and J.W. Sedat, Mapping the Architecture of
Interphase Nucleus.
A.J. Solari, Structural Analysis of Meiotic Chromosomes and
Synaptonemal Complexes in Higher Vertebrates.
J. Engebrecht, F. Klein, and J. Loidl, Genetic and Morphological
Approaches for the Analysis of Meiotic Chromosomes in Yeast.
M. Grote and R. Peters, Mapping Proteins to Nuclear Pore Complexes
by Immunogold Electron Microscopy.
R.L. Ochs, Methods to Study the Structure and Function of the
Nucleolus.
T. Kohwi-Shigematsu, I. DeBelle, L.A. Dickinson, S. Galande, and Y.
Kowi, Identification of Base-Unpairing Region (BUR)-Binding
Proteins and Characterization of their In Vivo Binding
Sequences.
Nuclear Assembly/Disassembly Cell-Free Systems:
B. Burke, Mammalian Systems to Study Nuclear Assembly.
M.J. Lohka and D.A. Warren, Analysis of Nuclear Envelope Assembly
Using Extracts of Xenopus Eggs.
P.A. Fisher and M. Berrios, Methods for Studying Nuclear Assembly
and Disassembly Using Drosophila Cell-Free Systems.
P. Collas and D.L. Poccia, Methods for Studying in Vitro Assembly
of Male Pronuclei Using Oocyte Extracts from Marine Invertebrates
Sea Urchins and Surf Clams.
Localization of Nuclear Processes:
R. Van Driel, E.M.M. Manders, L. de Jong, and J.A. Aten, Mapping
Sites of DNA Replication In Situ.
Y. Osheim and A.L. Beyer, Analysis of RNA Transcription and
Processing by Transmission Electron Microscopy.
G.H. Leno, Cell-Free Systems to Study Chromatin Remodeling.
C. Dingwall and I. Palacios, Reconstruction of Nuclear Protein and
snRNP Import In Vitro.
D.S. Goldfarb, Measuring Nuclear Transport Efficiency in
Saccharomyces cerevisiae Using a Nuclear Localization Signal-Tagged
Green Fluorescent Protein.
M.P. Terns and S. Goldfarb, The Nuclear Transport of Microinjected
RNAs in Xenopus Oocytes.
R. Benavente and G. Krohne, In Vivo Systems to Study the Dynamics
of Nuclear Lamins.
Y. Lazebnik, Apoptosis a Model System to Study the Structural
Collapse of the Nucleus.
Subject Index.
Key Features
* Provides a practical laboratory guide for studying cell
nuclei
* Includes comprehensive and easy-to-follow protocols
University of California, Santa Barbara, USA
"Nuclear Structure and Function is remarkably up to date, and all
of the authors have produced clear and very useful
contributions.
This handbook is highly recommended and will be essential for
researchers investigating all aspects of the nucleus, from the
biochemistry of specific processes through to nuclear architecture.
It will also be very helpful to all those who want to use some of
these techniques as part of their research, perhaps to investigate
the function of a particular gene product or the control of its
expression."
--TIBS
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