Table of Contents

The Biochemical Properties of Antibodies and their Fragments.- Hybridoma Technology.- Affinity Purification of Antibodies.- Bio conjugation of Antibodies to Horseradish Peroxidase (HRP). - Indirect ELISA.- Direct ELISA.- A Double-Sandwich ELISA for Identification of Monoclonal Antibodies Suitable for Sandwich Immunoassays.- ELISpot and DC-ELISspot Assay to Measure Frequency of Antigen-Specific IFNg Secreting Cells.- The Western Blot.- Development of Flow Through Assay for Detection of Antibodies to Taenia solium Using Protein-A Colloidal Gold Conjugate as a Probe.-Multiplexed Microsphere Suspension Array-Based Immunoassays.- Multiplex Immunoassay: A Planar Array on a Chip using the MagArray™ Technology.- Lateral Flow Immunoassay.- Immuno-PCR Assay for Sensitive Detection of Proteins in Real Time.- In-situ Proximity Ligation Assay (PLA).- Tyramide Signal Amplification for Immunofluorescent Enhancement.- Correlative microscopy for Localization of Proteins In Situ: Pre-Embedding Immuno-Electron Microscopy using FluoroNanogold, Gold Enhancement and Low-Temperature Resin.- tiplex ELISA using Oligonucleotide Tethered Antibodies.- Gas Plasma Surface Chemistry for Biological Assays.