SECTION I. BIOCHEMICAL, MOLECULAR, AND REAL-TIME DETECTION OF
NITRIC OXIDE
1. The preparation of anaerobic nitric oxide solutions for the
study of heme model systems in aqueous and non-aqueous media. Some
consequences of NOx impurities
2, The NOtizer – A device for the convenient preparation of
diazen-1-ium-1,2-diolates
3, Quantum Mechanical Determinations of Reaction Mechanisms, Acid
Base and Redox Properties of Nitrogen Oxides and their Donors
4, Electrochemical and spectrophotometric methods for evaluation of
NO dissociation rate constants from nitrosyl metal complexes
activated through reduction
5. Sensitive and isotope-selective (14NO/15NO) on-line detection of
nitric oxide by Faraday - Laser Magnetic Resonance Spectroscopy
(LMRS)
6. Qualitative and quantitative determination of nitrite and
nitrate with ion chromatography
7. Electrochemical Detection of Nitric Oxide in Biological
Fluids
8. Simultaneous Detection of NO and ROS by ESR in Biological
Systems
9. The ESR method to determine nitric oxide in plants
SECTION II. NITRATION AND S-NITROSYLATION
10, S-Nitrosothiol Formation
11. Detection and characterization of protein nitrosothiols
12. Detection of nitrosothiols and other nitroso species in vitro
and in cells
13. Detection and identification of S-nitrosylated proteins in
endothelial cells
14. S-Nitrosylation in Parkinson’s disease and related
neurodegenerative disorders
15. Dynamic assessment of nitration reactions in vivo
16. Protein nitration in biological aging: proteomic and tandem
mass spectrometric characterization of nitrated sites
17. HPLC-Electrochemical detection of tocopherol products as
indicators of reactive nitrogen intermediates
18. Hydrophobic tyrosyl probes for monitoring nitration reactions
in membranes
SECTION III: PEROXYNITRITE
19. Synthesis of peroxynitrite from nitrite and hydrogen
peroxide
20. Peroxynitrite-dependent upregulation of Src kinases in red
blood cells. Strategies to study the activation mechanisms
21. Hemoglobin and red blood cells as tools for studying
peroxynitrite biochemistry
22. Quantification of 3-nitrotyrosine levels using a bench top ion
trap mass spectrometry method
23. Mapping sites of tyrosine nitration by matrix-assisted laser
desorption/ionization mass spectrometry
24. Peroxynitrite in the pathogenesis of Parkinson’s disease and
the neuroprotective Role of metallothioneins
SECTION IV: SIGNALING AND GENE EXPRESSION
25. Yeast model system for examining nitrogen oxide
biochemistry/signaling
26. Fluorescence resonance energy transfer based assays for real
time detection of nitric oxide signaling
27. Nitric oxide is a signaling molecule that regulates gene
expression
28. NO signaling in ARE-mediated gene expression
29. Tyrosine phosphorylation in nitric oxide-mediated signaling
events
30. Identification and evaluation of NO regulated genes by
differential analysis of primary cDNA library expression
(DAzLE)
31. Role of NO in enhancing the expression of HO-1 in
LPS-stimulated macrophages
32. G protein signaling in iNOS gene expression
33. Determination of nitric oxide donor effects on tissue gene
expression in vivo using low density gene arrays
SECTION V: CELL BIOLOGY AND PHYSIOLOGY
34. Cell H2O2 steady-state concentration and mitochondrial nitric
oxide
35. Cytotoxic and cytoprotective actions of O2- and NO (ONOO-) are
determined both by cellular GSH level and HO activity in
macrophages
36. Determination of mitochondrial nitric oxide synthase
activity
37. Functional activity of mitochondrial nitric oxide synthase
38. Tetrahydrobiopterin as combined electron and proton donor in
nitric oxide biosynthesis: Cryogenic UV/Vis and EPR detection of
reaction intermediates
39. Antisense-mediated knock-down of iNOS expression in the
presence of cytokines
40. Soluble Guanylyl Cyclase: The nitric oxide receptor
41. Purification and characterization of NO-sensitive guanylyl
cyclase
42. The Measurement of Nitric Oxide Production by Cultured
Endothelial Cells
43. Use of microdialysis to study interstitial nitric oxide and
other reactive oxygen and nitrogen species in skeletal muscle
44. Nitric oxide, proteasomal function, and iron homeostasis:
Implications in aging and neurodegenerative diseases
45. Nitric oxide production in primary liver cells isolated from
amino acid diet fed rats
46. Update on nitric oxide-dependent vasodilation in human
subjects
47. Assessing NO-dependent vasodilatation using vessel bioassays at
defined oxygen tensions
48. Non-enzymatic nitric oxide formation during UVA-irradiation of
human skin: Experimental setups and ways to measure
49. In vivo evaluation of NO-dependent apoptosis by NMR
techniques
50. Microelectrode for in vivo real-time detection of NO
51. Real-time detection of nitric oxide isotopes in lung function
tests
52. ESR techniques for the detection of nitric oxide in in vivo and
in tissues
Final volume of the Nitric Oxide series in Methods in Enzymology
Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California. Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de l’Ordre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California. He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology: Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the “Antioxidant Network regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cell’s redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America.
"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE "It is a true 'methods' series, including almost every detail form basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY
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