Table of Contents

1: Russel, Lowman and Clackson: Introduction to phage biology and phage display
2: Sidhu and Weiss: Constructing phage display libraries by oligonucleotide-directed mutagenesis
3: Miyazaki and Arnold: In vitro DNA recombination
4: Dennis and Lowman: Phage selection strategies for improved affinity and specificity of proteins and peptides
5: DeLano and Cunningham: Rapid screening of phage displayed protein binding affinities by phage ELISA
6: Cwirla et al: Identification of novel ligands for receptors using recombinant peptide libraries
7: Matthews and Ballinger: Substrate phage display
8: Tuna et al: Protease-based selection of stably folded proteins and protein domains from phage display libraries
9: Isalan and Choo: Phage display of zinc fingers and other nucleic acid-binding motifs
10: Hoffman et al: In vivo and ex vivo selections using phage-displayed libraries
11: Monaci and Cortese: Screening phage libraries with sera
12: Jespers and Fransen: Interaction cloning using cDNA libraries displayed on phage
13: Bradbury and Marks: Phage antibody libraries
14: Neilson and Marks: Affinity maturation of phage antibodies

About the Author

Dr Tim Clackson
Vice President, Gene Therapy and Genomics
ARIAD Pharmaceuticals, Inc., 26 Landsdowne Street, Cambridge, MA 02139, USA
+1 617 494 0400 ext. 258
+1 617 225 2589
clackson@ariad.com
Tim Clackson, Ph.D. is Senior Vice President, Science and Technology at ARIAD Pharmaceuticals, Inc., where he has served for eight years in a variety of research and research management positions. His group is combining chemistry, cell biology and protein engineering to develop techniques for regulating gene expression and other intracellular processes using small molecules. Dr Clackson's particular research interest is the use of protein engineering and chemical biology techniques to analyze
and redesign the interfaces between molecules, especially small molecule-protein interfaces. Dr Henry B. Lowman
Senior Scientist
Genentech Inc., 1 DNA Way, South San Francisco, CA 94080, USA
+1 650 225 1171
+1 650 225 3734
hbl@gene.com
Henry B. Lowman, Ph.D. is a Senior Scientist and Associate Director of Antibody Engineering with joint appointments in Immunology and Protein Engineering, at Genentech, Inc., where he has been engaged in research on proteins and peptides for over thirteen years.