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Biofuels: Methods and Protocols
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Table of Contents

Biomass Supply Logistics and Infrastructure.- Selecting, Establishing, and Managing Switchgrass (Panicum virgatum) for Biofuels.- Agronomic Experiences with Miscanthus x giganteus in Illinois, USA.- Genetic Transformation of Switchgrass.- Lignocellulosic Biomass Pretreatment Using AFEX.- Pretreatment of Biomass by Aqueous Ammonia for Bioethanol Production.- Liquid Hot Water Pretreatment of Cellulosic Biomass.- Dilute Acid and Autohydrolysis Pretreatment.- Lime Pretreatment.- Analytical Characterization of Fermentation Inhibitors in Biomass Pretreatment Samples Using Liquid Chromatography, UV-Visible Spectroscopy, and Tandem Mass Spectrometry.- Biomass Compositional Analysis for Energy Applications.- High-Throughput Screening of Plant Cell-Wall Composition Using Pyrolysis Molecular Beam Mass Spectroscopy.- Preparation and Analysis of Biomass Lignins.- Cellulase Assays.- Assessing Cellulase Performance on Pretreated Lignocellulosic Biomass Using Saccharification and Fermentation-Based Protocols.- Basic Laboratory Culture Methods for Anaerobic Bacteria.- Simultaneous Saccharification and Fermentation and Partial Saccharification and Co-Fermentation of Lignocellulosic Biomass for Ethanol Production.- Biodiesel: Small Scale Production and Quality Requirements.

Reviews

From the reviews: “Bioenergy research, development and industrialization are moving at a rapid pace. … This book is therefore timely and focuses on lignocellulose-to-bioethanol technologies, with particular emphasis on maize residues and ‘energy crops’ … . Biomass supply, pretreatment and analysis are well covered … . This is a most useful text, or … lab handbook, that covers many analytical methods and applications for biomass-to-biofuel technologies.” (Graeme Walker, Microbiology Today, June, 2010)

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